![]() However, little is known about their functions in mammalian cells. gb file you are making.Demethylation by the AlkB dioxygenases represents an important mechanism for repair of N-alkylated nucleotides. ![]() fa file identified above and copy the sequence portion and then paste it on the line after ORIGIN in the bridging. Now that you have the ORIGIN signal that indicates a sequence follows, you are ready to paste in the sequence on the next line. Therefore on the line just above //, type ORIGIN and hit return. The sequence has to be prefixed by a signal. The sequence will be placed right before the // found at the end of the. Once it is saved you are ready to edit it safely. gb file as another name, preferably in a new directory. So that you don't destroy your UGENE files and projects, immediately save the. note="part of OMES-2, primer, rev primer for RPR1 region note="112 NUCLEOTIDES FLANKING RPR1 GENE" note="ADH1 terminator from S cerevisiae" note="portion of DNA from Arabidopsis with PRORP3 on it" note="Saccharomyces cerevisiae S288c chromosome XV" note="OMES-P1 primer, fwd primer for RPR1 region of note="28 nucleotides flanking insert (prefer 40)" It will look something like below: LOCUS Annotations 2
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